6×His Promotes Binding of a Recombinant Protein to Heparan Sulfate
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چکیده
منابع مشابه
6xHis promotes binding of a recombinant protein to heparan sulfate.
Commercially available systems provide a variety of strategies for the expression and purification of recombinant proteins. One popular method is the polyhistidine fusion tag, which usually consists of six consecutive histidines (6×His) added to the amino or carboxyl terminus of a protein (7). The high-affinity binding of 6×His to metalchelate resins allows easy purification of 6×His-tagged pro...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2002
ISSN: 0736-6205,1940-9818
DOI: 10.2144/02322bm04